Effects of Plant Hormones on Maturation of Secondary Somatic Embryos of Hevea brasiliensis

doi:10.11924/j.issn.1000-6850.casb15060136

Abstract

Abstract: In order to improve the regenerate rate of cyclic secondary somatic embryos in Hevea brasiliensis, establish high frequency regenerate system, the effects of NAA, GA3, 6-BA, KT and ABA on the maturation of long-term stored immature secondary somatic embryos were studied. The results showed that the effect of 0.05 mg/L NAA on the maturation of secondary somatic embryos was significant, 27.33 mature embryos were obtained on average. The optimal concentration of ABA was 1.0 mg/L, 24.33 mature embryos were obtained on average. Concentration changes of ABA could affect the number of mature embryos and root embryos. No secondary somatic embryos matured, and more radicle sprouted when the concentration of GA3 was low (0.2-0.4 mg/L). The best concentration of GA3 was 1.0 mg/L. High concentration of 6-BA and KT was disadvantage for the maturation of secondary somatic embryos, and the best combined concentration was 0.5 mg/L 6-BA and 1.0 mg/L KT.

CLC Number:

Q813.1

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