Abstract: Lycopersicon esculentum ‘Xiariyangguang’is an imported high- level tomato variety recently, which gets the favor of market because of high yield and high quality. However, it is difficult to be planted in large scale for marketing because of its expensive seeds, which are all hybrid F1 generation. Base on the plant in vitro culture techniques, the seedlings ofLycopersicon esculentum‘Xiariyangguang’will be propagated rapidly. Therefore, it is a feasible way for promoting this variety in production. In order to rapidly establish aseptic lines ofLycopersicon esculentum‘Xiariyangguang’and obtain high-performance proliferation of its shootsin vitro , the key techniques were investigated by using its lateral buds as materials during itsin vitro culture. The results showed that after its explant was disinfected by using 0.1% mercuric chloride with some Tween 20 treatment for 8 min, the aseptic lines of Lycopersicon esculentum ‘Xiariyangguang’could be established in media MS 0.1 mg/L BA 0.1 mg/L IBA, whose seedling rate was 46.7%. The medium MS 0.5 mg/L BA 0.1 mg/L IBA was suitable for bud seedling subculture and proliferation to obtain healthy and strong plantlets. Its multiplication rate could reach 4.3. These techniques could lay the foundation for largescale propagation ofLycopersicon sculentum‘Xiariyangguang’seedlings.