Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2007, Vol. 23 ›› Issue (9): 7-7.

Special Issue: 生物技术 畜牧兽医

• 畜牧兽医科学 • Previous Articles     Next Articles

Amplifying and Sequencing Analysis of ORF1 Gene of ZZ Strain Porcine Circovirus Type 2

Wang Yan, Wang Xianwen, Hou Chunbing, Wang Xinwei   

  • Online:2007-09-05 Published:2007-09-05

Abstract: The ORF1 gene was amplified from from ZZ strain of PCV-2 respectively using the primers designed according to the PCV-2 OFR1 sequence published in Genbank. The nucleotide sequence data of PCV-2 ORF1 gene was analyzed by comparing with that of other PCV-2 ORF1 gene sequence. The results indicated that the nucleotide homology of all selected PCV-2 ORF2 genes was 97.3 %~100 %.Meanwhile, several conserved founctional domains were discovered in PCV-2 ORF1 gene and found the protein translated by PCV2-ORF1 gene having well immunogenicity.

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