Abstract: 【OBJECTIVEI】In order to establish the base of the peach dwarfing rootstock’s isolated system, compared the disinfectant effects on the peach dwarfing rootstock Yunong 1, looking for the reasonable and effective disinfectant method. 【METHOD】 The stem segments of the dwarf Yunong rootstock No.1 were disinfected by 75% C2H5OH、HgCl2 、NaClO and disinfectants . 【CONCLUSION】The results indicated that by compared the disinfectant effect, it can be concluded that 75% C2H5OH matched disinfectants was obviously better than 75% C2H5OH matched NaClO or HgCl2. As the result , using 75% C2H5OH 30s+1.5g/L disinfectant 20 minutes was the best method.