Optimization of SRAP Reaction System in Cassava

Abstract

Abstract: The aim of the research was to establish SRAP amplification system which was suitable for Cassava DNA so as to lay foundation for molecular marker and the construction of gene map in Cassava. Using Cassava genome DNA as template, sequence-related amplified polymorphism (SRAP) was applied for Cassava to carry on PCR amplification of its DNA and optimize the reaction parameter grade by grade. The stable and reproducible SRAP reaction system of Cassava has been developed. The optimum SRAP PCR system (total of 10μl) was as follows: DNA (50ng/μl) 0.5μl, 10×PCR buffer (Mg2+) 1.0μl, dNTPs (20mM) 0.2μl, primer (50ng) 0.3μl, Taq polymerase (5U/μl) 0.2μl. The program and system could meet the demands for genome SRAP amplification in Cassava. It was feasible to apply SRAP marker in genetic research in Cassava.

CLC Number:

Q789

Xia Zhiqiang, Zou Meiling, Wang Wenquan. Optimization of SRAP Reaction System in Cassava[J]. Chinese Agricultural Science Bulletin, 2008, 24(9): 457-460.

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