Abstract: This experiment attempted to research the separating effect of coconut storage protein subunits under different separation gel concentrations of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the separating effect of coconut storage protein subunits could be greatly affected by the concentration change of separation gel. The good separating effect of coconut storage protein could be obtained when the separation gel concentration was 11% to 12%. The resolving effect of coconut protein subunits was best when the separation concentration was 11.5%.