Abstract: To establish the high regeneration system of Kiwifruit in vitro culture the stem segment and apex explants in Actinidia was studied in this paper. After having gained in vitro plantlet successfully different of hormonal concentration combinations were added in MS and the 1/2 MS medium to study the effect of hormonal combinations with different concentrations on shoot induction and root induction. In initial culture of stem segment the plantlet had been gained successfully and grown well in the medium of MS +1.0 mg•L-1 6-BA +0.1 mg•L-1NAA. In the medium of MS +2.0 mg•L-1 6-BA +0.1 mg•L-1 NAA the rates of induction budding from leaf and petiole were 1.38， 5.13 respectively. The results showed that concentrations of IBA was 0.9 mg•L-1 and 0.6 mg•L-1 added in 1/2 MS in rooting culture medium were effective to shoot in vitro rooting， of which the total root length， the average root length and root number was higher than other treatments. The survive rate of plantlet transplanted into the substrate composed of 30% perlite and 70 % peat-moss was 80%.The result of this study has laid a foundation for rapid propagation in kiwifruit.