Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (10): 43-46.
Special Issue: 生物技术; 园艺
• 畜牧兽医科学 • Previous Articles Next Articles
Yi-ping Du,Ling Zou,WENHUA LIU
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Abstract: In order to investigate the situation of methicillin-resistant Staphylococcus(MRS) in livestock and poultry, the method of PCR and dig-labeled nucleic acid probe to mecA gene fragment were established to detect 75 Staphylococcus isolates. Forty-two strains of MRS were screened out by PCR, accounting for 56.0%. Forty-seven strains of MRS were screened out by nucleic acid probe method, accounting for 62.7%. The coincidence rate of acid probe method with PCR method was 93.3%. It was verified that MRS could be isolated from livestock and poultry in vivo or in vitro, with higher isolation rate. Veterinary researchers should pay more attention to Methicillin-resistant Staphylococcus.
Yi-ping Du,Ling Zou,WENHUA LIU. Detection of mecA Gene of Methicillin-resistant Staphylococcus by PCR and Nucleic Acid Probe[J]. Chinese Agricultural Science Bulletin, 2008, 24(10): 43-46.
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https://www.casb.org.cn/EN/Y2008/V24/I10/43