Abstract: The coding sequence of porcine fat specific protein27 gene were amplified based on the in silico sequence information in the present study. Total RNA was extracted from all kinds of organizations and mRNA sequence of gene were amplified by RT-PCR using designed primers. The PCR products were ligated into the pMD-19Tvector, and then transformed into competent cells of E. coli JM109. Porcine fat specific protein27 gene cDNA sequence, the 745 bp in length, open reading frame was 11-727 bp detected by RT-PCR,encoding 238 amino acids residue protein. Homology analysis showed that porcine FSP27 and nucleic acid sequences human, mice and bovine were the homology of 86%, 77.6% and 82.3% amino acid sequence homology was 83.2%, 74.4%and 79.3%. The results showed that tissue distribution: Pig in a variety of organizations have FSP27 distribution.Cloning porcines FSP27 genes and registration GenBank (Accession.EU395789).