Chinese Agricultural Science Bulletin ›› 2008, Vol. 24 ›› Issue (11): 78-80.
Special Issue: 生物技术； 小麦
• 农业生物技术科学 • Previous Articles Next Articles
Meng Fanrong, Si Zhifei, Liu Haoying, Zhang Wen
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Abstract: In this study, a prokaryotic expression vector pGEX-4T-MBD3 for the wheat MBD (Methyl-binding domain protein) gene MBD3 was constructed. Transform it to E. coli. BL21 (DE3), the fusion protein GST-MBD3 with molecular weight of 49.6 kDа was effectively expressed under 1mM IPTG concentrations at 37°C, which provides a foundation for further purification and functional study of the MBD3 protein.
Meng Fanrong, Si Zhifei, Liu Haoying, Zhang Wen. Recombinant expression vector with wheat MBD3 gene and its prokaryotic expression[J]. Chinese Agricultural Science Bulletin, 2008, 24(11): 78-80.
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https://www.casb.org.cn/EN/Y2008/V24/I11/78
