Abstract: Abstract: The primary cultured endometrial cells in rabbits were isolated and purified by collagenase digestion method, cultured in vitro, and induced inflammation by lipopolysaccharide (LPS). The morphology and traditional inflammation factors of the cells were measured as the development criteria of inflammation. The cells were treated with gradient concentrations of LPS, the culture supernatants were collected in different period, and the contents of TNF-α, IL-1 β were determined by ELISA. The results showed that 100 ng/mL LPS was the optimal concentration to induce the inflammation of cultured endometrial cells. The supernatant TNF-α, IL-1 β in the inflammatory model group were higher than those in control at the same period (P<0.01), the inflammatory model of the endometrial cells was created successfully.