Abstract: Abstract: To obtain the open reading frame(ORF) of Hsc70t gene in mouse testis, the specific primers were designed according to Hsc70t gene order published in GenBank（GenBank accession No:NM_013558）, The total DNA was extracted from mouse testis, The Hsc70t gene was amplified by PCR from total DNA,and connectioned to pMD18-T vector , then sequencing and analysis were accomplished. Sequencing result showed the sequence contained 1926bp was obtained by PCR amplification. The sequence was embodied by GenBank, the Genbank accession number is EU549780, The similarity of nucleotide sequence was 99.9% approximately between the cloned gene and the mouse testis Hsc70t gene in Genbank, Comparison of the nucleotide sequence between mouse and the Rattus norvegicus, the Macaca mulatta, the Pan troglodytes, the Xenopus, the Homo, the Oryzias latipes by DNA Star, shows similarity score were 94.4％、85.4％、84.9％、72.7％、71.9％、67.1％, Phylogenetic analysis showed mouse had the closest genetic relationship with rattus norvegicus. The results confirmed that the Hsp70 has highly conservatism in different organisms and this trial provides a good foundation for further research and application of Hsc70t