Abstract:

The changes of Ca2+-ATPase activities and EPN contents in oyster were studied during storage at three different temperatures（-10±1℃、-15±1℃、-20±1℃）. The kinetics models of protein denaturation were established to correlate protein denaturation rate with temperature and time, and the changing equation of oysters’ protein denaturation system was set up during frozen storage. Results showed that: The changes of Ca2+-ATPase activities and EPN contents accorded with first order reaction kinetics model, and protein denaturation was related to temperature and time during frozen storage. The decreasing tendency of Ca2+-ATPase activities and EPN contents was uniform with the increasing of temperature and time. The result of protein denaturation was less at lower temperature than that at higher one. The changing equation of protein denaturation could describe the changes of frozen oysters’ qualities effectively within the range of experiment.