Abstract: Mature waxy maize embryos were cultured as explants in vitro, and the effects on callus induction and subculturing were investigated under culture mediums with different additives, such as plant hormones, AgNO3, organic compounds, etc. The results showed that the combination of N6 + 0.2mg/L 6-BA + 2mg/L 2,4-D could obtain the most calli of higher quality in the induction phase, while the combination of MS + 0.2mg/L 6-BA + 2mg/L 2,4-D +10mg/L AgNO3 or 10% CW could make sure the calli of higher quality further in the stage of subculturing. Key words: Waxy maize, Mature embryo, Embryogenic callus