Abstract: Having studied the biochemical index of germinating of the two breeds of cole in an artificial climate box, Aijiaohuang and Aikangqing, I have found that with the increasing concentration of the menace of Cu ion solution, the higher the exosmosis rate is, and the electrolyte exosmosis rate also rises gradually; that the cole seeds bred in the lower concentration of Cu menace contains more dissoluble glycogen, and with the increasing concentration of Cu ion solution, the dissoluble glycogen of the cole seeds is on the decrease; that the total amount of amylase and the activity of α-enzyme of the cole seeds are the highest level when the concentration of Cu ion solution is at 40μM, then, with the increase of the concentration, the activity of enzyme declines apparently; that the content and the accumulation of MDA in the seed are both on increase with the increasing of the concentration Cu ion solution.