Abstract: To screen and identify flue-cured tobacco germplasms, botany characters, appearance quality, disease resistance and economical characters of 20 tobacco germplasm was investigated. The results indicated that the plant height of Liuyejian and Longyan C2 was too high, the leaves of Changbahuangguangban, Xujin 7, Black Shank Resistant, Daris Special, Zhubo1was too few, Liuyejian, Xujin 7, Black Shank Resistant and Daris Special was susceptible to tobacco black shank disease, Daliuye 2014, NC2514, Daris Special, Luodihuang was susceptible to tobacco brown spot disease. By comparation in economical characters and other aspect it was concluded that Islangold, CV78-4, CV78-5, Q1236:Hicks Q46, Wujiang 1 was superior to other tested germplasms. Daris Special, Black Shank Resistant, Changbahuangguangban, Jintai 7615, Daliuye 2014was inferior to other tested germplasms.