Abstract: In order to clarify the relation between the endogenous salicylic acid content of strawberry fruit and its diseases resistance, and to help estimating the amount of SA taken from fruit comsumptioin by people, HPLC was used to measure the SA content of five varieties of strawberry which are susceptible or resistant to powdery mildew in open field and plastic simple greenhouse. The results showed that SA contents are greatly different among five strawberry varieties in two cultivation conditions, 1.904µg/g～5.023µg/g in open field and 1.068µg/g～4.201µg/g in plastic simple greenhouse respectively. There is no correlative relationship between endogenous SA contents in the fruits and their resistant ability against powdery mildew. Bound SA was the predominant form of endogenous SA in strawberry fruits with being 4 to 20 times as high as free SA in the measured five varieties of strawberry fruits. Both endogenous free SA and bound SA of strawberry fruit were declined gradually with the fruit maturity. It is suggested that SA content in strawberry fruits is controlled not only by genotype, but also by the cultivation and the environment.