Observation of the Vitro bacteriostasis Effects of Chinese Herbal Medicine on the Major Pathogenic Bacteria Causing Mastitis

Abstract

Abstract: Three kinds of major pathogenic microorganisms that cause mastitis of diary cows were selected and the in vitro antibiosis effects of 21 kinds of Chinese medicinal herbs such as Radix Scutellariae et.al. were tested through the method of stiletto. Twenty four nutrient agar medium were divided into there groups. Smear Escherichia Coli on A1-A8 nutrient agar medium homogeneous. Smear S. aurous on B1-B8 nutrient agar medium homogeneous. Smear Streptococcus agalactiae on C1-C8 nutrient agar medium homogeneous. Then slotting uniform upon the nutrient agar medium, respectively, each plate six hole. Add 20μL of every medical solution into each hole, and two holes were made to parallel samples. Add 21 chinese medicine to A1-A7，B1-B7，C1-C7, respectively,and A8,B8,C8 were the normal control which were added deionized water only. These nutrient agar medium were incubated at 37℃ for 24 h. Bacterial existence and antibacterial circle diameter were observed. We found that different medicine for different strains produce different effects.The experiment results showed above supplied the scientific evidence for filtering Chinese medicinal herbs to prevent and cure the mastitis.

CLC Number:

S853.74

Zhang Shaohua, Sun Dandan, Shi Wanyu, Qin Jianhua, Zhong Xiuhui, Bao Yongzhan, Ren liqin. Observation of the Vitro bacteriostasis Effects of Chinese Herbal Medicine on the Major Pathogenic Bacteria Causing Mastitis[J]. Chinese Agricultural Science Bulletin, 2009, 25(14): 11-14.

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