Abstract: The relationship between physiological-biochemical changes and drought resistance of different wildness Rose provenances were studied. The provenances were selated from Jilin Huicun, Shandong Muping, Shandong Rongchen.The results showed that the tendency of the physiological and biochemical indexes in three provenances under drought stress were similar. And the change degree of the indexes were different due to different sources of provenances. Through coordinate composite evaluation on 5 physiological and biochemical indexes, the results indicated that drought-resistance sequence of three provenances were Jilin Huicun, Shandong Muping, Shandong Rongchen.