Abstract: The results of making transparence specimen of mouse skeleton were compared respectively, making mouse death in 4 different methods and dyeing them in 3 methods. The results shows, drown or etherization to make mouse death, Alcohol-fixed, defatted with 3% potassium dichromate, vitrification with Potassium hydroxide, coloration of bone with Alizarin Red S, the glycerol-ammonia were respectively coloration of cartilage with Alcian blue, vitrification with glycerol which had different concentrations, were the best conditions for making transparent specimen. Temperature controlling could reduce the time of specimen-fixed.