Abstract: The active ingredients were extracted from several Chinese tradional medicine by water ( for mulberry leaves, yinchenhao, portulaca oleracea ) or alcohol ( for wild chrysanthemum ) and their effects on reproduction of avian influenza virus (AIV), Newcastle disease virus (NDV) and avian infectious bronchitis virus(IBV) in chicken embryo were observed evaluated. The results showed that extracts from these Chinese tradional medicine possess certain blocking and inhibitory effect on these viruses reproduction within the range of safe concentration, especially the extract from Yinchenhao on AIV and NDV.