Abstract:

Abstract: Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) could grow in PAM, the cell line of CL2621 and Marc-145, but CPE is not distinctness, and the replication disciplinarian is still clearly unknown. This experiment investigated that the replication disciplinarian of PRRSV in the line of Marc-145 using Fluorescence Quantitative PCR. The results found that the virus begin to replicate in a great speed at the 12 h after inoculation and its growth is fastest between the 18-36 h, reaches peak at 48 h. These experiments supplied the production of vaccine and helps to do research on viral pathology of PRRSV.