Abstract:

The purpose of this study was to optimize the PCR reaction system of molecular technique for detecting the pig component in the food and feed. The PCR reaction system of 20μL was used by this test . The PCR reaction system was optimized when the Taq DNA polymerase are 0U、1U、2U、3U, and the MgCl2 are 0μL、2μL、4μL、6μL, and the dNTP are 0μL、0.1μL、0.2μL、0.4μL and the other reaction factors of PCR reaction system are the maximum. The result is that :1、the fitting concentrations for the Taq DNA polymerase, 25mMol?L-1 MgCl2 and dNTP in the PCR reaction system are 2U, 2μL and 0.4μL respectively; 2、the minimum concentration of DNA of pig components in this optimizational PCR reaction system can be detected is 0.04 ng?μL-1. The conclusion: The optimal PCR reaction system for the pig component which has been validated by the Duplex PCR technique showes that it can work accurately and efficiently.