Abstract:

A total length DFR gene was cloned with RT-PCR and RACE technology from flower bud with blue petals of Hyacinthus orientalis L.. Hyacinthus DFR cDNA was 1252 bp in length.Within the f ull-length cDNA , a clear open reading frame (ORF) was 1098 nucleotides ,coding 366 amino acids. The result s of homologous analysis in GenBank demonstrated that the sequence had 66 %～77 % identity on the nucleotide sequence and 62 %～73% identity on the deduced amino acid sequence. The results of phylogenetic analysis showed that DFR from Hyacinthus orientalis and from Iris x hollandica clustered together firstly ,and then came those from other monocotyledon, dicotyledon came subsequently.