Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (8): 69-73.
Special Issue: 生物技术； 园艺
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Li Juanling1,2, Liu Guomin1,2, Jia Yuan2, Lan Yunfeng2
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Abstract:
Based on the routine CTAB method, some steps and reagents used to obtain DNA, were modified in this study to develop a simple and rapid method for removing the interference of some chemicals, such as polyphenols, anthraquinones, sterids and so on. The molecular weight of DNA, isolated by modified CTAB method, was nearly 21 kb, and the OD260/OD280 was about 1.8. Using the DNA isolated by modified CTAB method as the template in ISSR-PCR, the excellent amplification result, with distinct band and fine repeatability, could be obtained.
Li Juanling1,2, Liu Guomin1,2, Jia Yuan2, Lan Yunfeng2. An Effective Method for Genomic DNA Extraction in the Mallotus Oblongiolus(Miq.) Muell.-Arg.[J]. Chinese Agricultural Science Bulletin, 2010, 26(8): 69-73.
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https://www.casb.org.cn/EN/Y2010/V26/I8/69
