Abstract:

Effect of media, hormones, pH, dark incubation time, the stem site and different sealing materials on the regeneration of Vaccinium angustifdium ‘Blomidon’stem were studied.The results showed that: the appropriate medium was WPM; ZT 1.0 mg ? L-1 induced stem regenerated plants without callus was the best, ZT 5.0 mg ? L-1 + IBA 1.0 mg ? L-1 induced by the callus regenerated plants was best, regeneration frequencies were 100% and 91.67%; suitable pH was 5.0 ± 0.2; suitable dark time was 0 d or 10 d; the upper part of stem was the best part for vaccination; the cellophane was suitable sealing material.