Abstract:

The mecA fragment which encodes the transpeptidase domain of penicillin binding protein 2a(PBP2a)of methicillin resistant staphylococcus aureus(MRSA),to express and identificated the transpeptidase domain of PBP2a by western blot. The mecA fragment which encodes the transpeptidase domain of PBP2a was amplified from the genomic DNA of MRSA by PCR, and then the objective gene fragment was cloned into pGEX-6p-1 plasmid. The recombinant plasmid pGEX-6p-1-TPase was transformed into BL21.The expression was induced by IPTG and the expressed product was identificated by western blot.The results indicated that the corresponding prokaryotic expression vector was successfully constructed, and the transpeptidase domain of PBP2a was successfully expressed, which established the foundation for further investigation.