Abstract:

The standard sera Haemophilus parasuis(Hps) , which were isolated and identified by Dr. Cai Xue-wang of Huazhong Agricultural University, were cultured, killed and immuned rabbits twice. The rabbits were challenged by ear vein injection and blood from heart. The antibody titers were detected by Gel Diffusion (GD) test. The rabbits were blood by jugular vein if the titers were more than 1:8. The serum were separated and used to detected the serotype of Hps which were isolated from Henan pig farms by the lab with GD method. There are 12 strains serovar 4, 9 strains serovar 5, 6 strains 13, 2 strains serovar 14 and 1 strains serovar 2, and no other serovar among 33 isolates. The results showed that serovar 4, 5, 13 were the major prevalent serotype in Henan.