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Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (11): 261-263.

Special Issue: 玉米

• 23 • Previous Articles     Next Articles

Identification of the Pathogen of Maize Alternaria leaf spot

Zhou Shuyang1, Wang Chunlei1, Qiao Zhixin2, Yang Hongyi1   

  • Received:2010-01-06 Revised:2010-01-11 Online:2010-06-05 Published:2010-06-05

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Abstract:

The pathogen of Maize Alternaria leaf spot was isolated and purified, and according to Koch's postulate, Alternaria leaf spot of maize was caused by the isolated fungi. The internal transcribed spacer (ITS) region of pathogen was amplified by polymerase chain reaction (PCR), and the specific fragment was cloned and sequenced. The result showed that the sequence of ITS for the pathogen shared 99% homology with that of Alternaria tenuis in GenBank. The pathogen was identified as Alternaria tenuis based on the morphological characteristics and sequence of ITS.