Abstract:

In order to protect the wild resources of Dendrobium huoshanense in better and change their home of wild species. In vitro shoots of Dendrobium huoshanense test material, using three factors, three level L9 (33) orthogonal design to study BA, NAA, KT of different hormones and concentration on the propagation of adventitious buds of Dendrobium huoshanense subculture proliferation. The results showed that: Plant hormones BA, NAA, KT right Dendrobium huoshanense proliferation of in vitro multiple shoot clumps have a certain impact, in which the impact of NAA and KT even more pronounced and, through the SAS statistical analysis software to determine the optimal concentration of three kinds of plant hormones ratio are as follows: BA use 1.5 mg / L, NAA use 1.0 mg / L, KT selected 1.0 mg / L, Namely, the best medium formula of Dendrobium huoshanense subculture in vitro multiple shoot clumps as follows: MS+BA 1.5 mg/L+NAA 1.0 mg/L+KT 1.0 mg/L+ Sugar 30.0 g/L+ Agar 8.0 g/L。