Abstract: Endophytic fungi strains were isolated from stem and leaf of wheat. According to the ability to inhibit asexual spore germination on bearing glass, 4 isolates with antagonism were singled out and their control efficiency achieve to 86.08%, 83.12%, 91.14% and 96.23%, respectively. Fermentation filtrate of 4 strains were used to curb the disease development on detached wheat leaves. State of illness was identified according to the six standard and the Ind (%) were calculated. The results showed that BF11 and BF19 had better preventing efficacy for Blumeria graminis. The state of illness about wheat powdery mildew alleviated and biocontrol efficacy of BF11 and BF19 were 82.14% and 88.49%. The characteristics of morphology suggested that the strain of BF11 was identified as Fusarium and BF19 was identified as Aspergillus.