Abstract: the flammulina velutipes mycelium growth rate and the carbon-nitrogen ratio is proportional in the mediumbetween the ratio of 25:1 ~ 60:1 ratio, while when the carbon-nitrogen ratio is greater than 40:1, with the higher carbon-nitrogen ratio of material culture, the flammulina tends to have less output with the increase of the carbon-nitrogen ratio . Carbon and nitrogen analysis showed that the material culture of Flammulina velutipes of protein nutritional is irrelevant with the Carbon and nitrogen proportions in the training materials while it has impacts on the mycelium growth rate and output of the Flammulina velutipes .