Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (21): 268-272.
Special Issue: 生物技术; 智慧农业
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Abstract: Wuyiencin, an agricultural antibiotic, was produced from the Streptomyces ahygroscopicus var. wuyiensis. Wuyiencin used as fungicide has strong inhibitant effects and obvious control effects on many fungi pathogenic of crops. The aim of this research is to increase production of producing strains and decrease production costs of Wuyiencin. By using Plackett-Burman Design and Response Surface Method which provided by SAS software to optimize the fermentation culture medium of strain C-75.Corn flour and CaCO3 were chosen as the major components from nine compositions of medium. Then the regression model of RSA was established by Response Surface Method: Y=5826.99-91.56X2-180.69X8- 194.31X22-151.25X2X8-205.31X82.It was predicted that when the concentrations of corn flour and CaCO3 were 2.314g and 0.205g respectively(the volume of medium is 50ml), the regression model exist maximum. The average tieter of Wuyiencin was determined 5837μg/ml actually by shake-flask. The Wuyiencin production was increased by 38.53% when strain C-75 was cultivated in the optimum medium instead of the original one.
CLC Number:
S482.7
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https://www.casb.org.cn/EN/Y2010/V26/I21/268