Abstract:

Sample preparation is the most basic and critical link in two-dimensional electrophoresis experiment.The success of sample preparation is directly related to the success of the quality of two-dimensional electrophoresis map and the integrity of proteome information.The commonly extraction methods of plant total protein applied to two-dimensional electrophoresis: TCA-acetone precipitation method, Tris-Hcl method, phenol method, Trizol precipitation method, Tris-acetone - phenol method, Urea/Thiourea extraction. Different method is suitable for different characteristics of plant samples, Compare the advantages and disadvantages of the six kinds protein extraction methods of two-dimensional electrophoresis, and gave a brief overview of each method applicable to plant tissues.