Abstract:

Abstract: Two pepper hybrid varieties, Hangjiao No.4 and Hangjiao No.5, and their parental inbred lines H09-4、H09-2、WS-3、B-2-2 were used to study the polymorphorism of amplifiable spectral bands in hybrids and their parents by SRAP marker in order to detect true and fake hybrid seeds. The 18 pairs of tested SRAP primers, there were 13 pairs of primers found to be polymorphic in amplification in the two hybrid varieties, Among18 primer pairs, 23.5 strong markers were produced for every primer pair on the average, the average polymorphic ratio was 58?89%, 3 primers of partial female type, 2 primers of partial male type, 2 primers of complementary type. 100 seeds of Hangjiao No.4 and Hangjiao No.5 were individually tested with primers DC1-EM9 and ME5-EM20 to have a purity of 100% and a purity of 97.3%, respectively, and the purities were very close to the purities field tested 100% and 98.9, which indicated that SRAP markers would have a wide range of applications in rapid lab test of seed purities of watermelon hybrid varieties