Abstract:

Abstract: MPBQ-MT (2-methyl-6-phytyl-1,4-benzoquinone methyltransferase，VTE3) , one of key enzymes in vitamin E pathway, catalyses 2-methyl -6- phytyl-1,4- benzoquinone (MPBQ) to synthesis 2,3-dimethyl-6 -phytyl-1,4- benzoquinone (DMPBQ) by adding a methyl.. Soybean VTE3 (GmVTE3) gene was inserted into the plant expression vector and transfered into Arabidopsis thaliana. The Vitamin E contents from transgenic Arabidopsis seeds and leaves were analyzed using HPLC. The results showed that the ratio of γ-tocopherol / δ-tocopherol increased by 1.7 times in transgenic Arabidopsis seeds than the wild-type and the δ-tocopherol was undetectable in leaves, demonstrating GmVTE3 gene effectively transferred the δ-tocopherol into γ-tocopherol in the leaves and seeds of transgenic Arabidopsis.