Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (20): 47-51.

Special Issue: 生物技术 园艺

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DNA Fingerprinting Analysis of Varieties in Cucumis melo ssp. melo

  

  • Received:2010-04-22 Revised:2010-05-18 Online:2010-10-20 Published:2010-10-20

Abstract:

Simple sequence repeat (SSR) markers were used to construct DNA fingerprinting among 21 accessions of Cucumis melo ssp. Melo, which could provide a possibility of establishment of DUS and accession identification of Cucumis melo ssp. Melo. 23 polymorphic SSR primers were screened from 700 primers, they generated 63 polymorphic bands with 2.74 alleles per primer, the polymorphism information content (PIC) varied from 0.52 to 0.69, with an average of 0.61. The SSR fingerprint maps of 21 varieties were established according to the SSR characteristic bands identified by six primers combinations (CMGAN12,MU7161,F22_85,F21_16,ECM79 and MU5499), it will provide reliable scientific evidence for the protection of intellectual property right for these cultivars.