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Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (20): 38-42.

Special Issue: 园艺

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GENETIC ANALYSIS AND MOLECULAR LOCALIZATION OF THE FRUIT DIAMETER IN CUCUMBER

  

  • Received:2010-05-17 Revised:2010-06-16 Online:2010-10-20 Published:2010-10-20

Abstract:

In this study, selected big fruit diameter of D07171 and D0899, small fruit diameter D0435-3-1 and D0438-3, in accordance with the method of complete diallel crosses have been the preparation of 6 combinations to Analyze the Genetic model of fruit diameter. The combination of D0435-3-1×D07171 was selected as the largest different combination to get the F2 generation for test of normality, using SSR molecular marker technology to build genetic linkage map. Cucumber fruit diameter of many genes are controlled by the quantity of genetic. Cucumber fruit diameter in line with the additive-dominance (AD) genetic model for the additive effect of the main, dominant effect is less than additive effect. The broad-sense heritability and narrow sense heritability of the cucumber fruit diameter are both high, 60.64% and 57.26% respectively. In this study, 116 pairs of SSR primers in the DNA of two parents and between the pool filter, detected with a cucumber fruit diameter a QTL related sites, located in the genetic map of linkage group CSWCT25-CSWCT29-CSWTA03, and CSWTA03 closer distance for the 1.98 cM , the corresponding LOD value of 3.22, 7.19% explain the genetic variation.