Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (20): 15-17.

Special Issue: 畜牧兽医

• 23 • Previous Articles     Next Articles

Rapid Detection of Riemerella anatipestifer infection by Loop-Mediated Isothermal Amplification Assay

  

  • Received:2010-05-19 Revised:2010-06-21 Online:2010-10-20 Published:2010-10-20

Abstract:

According to the ompA gene of Riemerella anatipestifer (RA), a set of specific primers were designed to amplify the special DNA sequences by LAMP. Moreover, the reaction conditions of LAMP were optimized. The results showed that the optimal reaction temperature and reaction time were 65℃ and 80 min, respectively.