Abstract:

Gene polymorphisms include lots of genetic information. Typing technology is the main method to evaluate gene polymorphism. The parallel typing system based on ligase detection reaction was established in the present study, and to detect the polymorphism of NPY gene, as well as its genetic effect on age at first laying, egg weight at first laying, body weight at first laying, average egg weight at the age of 40 weeks, average egg weight at the age of 50 weeks, and total egg number at the age of 52 weeks in Qingyuan partridge chicken. The genotypes of 4 bp deletion mutation in NPY transcription start region were identified in Qingyuan partridge chickens, and the results were coincidence with direct sequencing. The least square analysis showed that only body weight at first laying has significantly difference between different genotypes. BB genotype birds had significant lighter body weight at first laying than AA and AB genotype birds