Abstract:

In order to exploit application resources of wild mushroom, we collected four fruiting bodies of wild mushroom which are common in Peking mountainous areas, Pnoliota adiposa, Chroogomphis rutillus, Suillus brevipes and Lasiosphaera fenzlii. We detected the contents of protein and hydrophilic polysaccharide, proteinase, phosphatase, laccase and lectin in four types of fruiting bodies by bicinchoninic acid method, phenol-sulphuric acid method and modified enzyme detection. The aim of this study is to compare the resource value of the four wild mushrooms, and find the wild materials for further processing. The results showed that the contents of protein and hydrophilic polysaccharide of Pnoliota adipose, which were equal to 5.12% of fresh mycelium weight and 2.39% of dry mycelium weight, respectively, were higher than those of the others. Lectin activity was only found in the fruiting body of Pnoliota adipose. The result showed that all of the tested wild mushroom exhibited the phosphatase activity, and the activity of Lasiosphaera fenzlii was the highest at 339.91 U/mg. Suillus brevipes and Lasiosphaera fenzlii were found with slight laccase activity at of 0.341 U/mg and 0.438 U/mg, respectively. Proteinase activity was not found in the tested mushroom. All in all, Pnoliota adipose, with high protein and polysaccharide contents, as well as Lasiosphaera fenzlii, with high phosphatase activity, can be considered as advanced materials in process investigation.