Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2005, Vol. 21 ›› Issue (2): 43-43.

Special Issue: 油料作物 园艺

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The proplast isolation and purification of yellow-seed Rape

Fan Chunli, Tao Lan, Lin Na, Wu Xiaoliang   

  • Online:2005-02-05 Published:2005-02-05

Abstract: Proplasts were isolated and purified from hypocotyls and plantlet of two yellow-seed (Brassica napus L.) cultivars SH21 and Yuhuang No.1 .Low temperature treatment during seed generation could apparently increase the vigour of protoplasts. The yield of protoplasts was effect by different enzyme. The method of purification called filtrate-centrifugl-float, floated by 21%sucrose and centrifugalized at 800 r/min is better.