Abstract:

The callus was induced from the leaves of Portulaca oleracea L., and the callus was used for the receptor of genetic transformation mediated by Agrobacterium tumefaciens. The resistant callus was screened and confirmed to be transformants by GUS histochemical assays and PCR. It showed that genetic transformation of Portulaca oleracea L. callus mediated by Agrobacterium tumefaciens was entirely feasible.