Abstract:

In order to improve salt tolerance ability of poplar, in the present study, according to the restriction enzyme site of pGN vector and the tonoplast H+-PPase gene LcVP1 cDNA sequence from Lotus corniculatus L, a pair of primers containing restriction enzyme site was desigined and used to amplify LcVP1 ORF. PCR pruducts and the plasmid pGN were digested by the corresponding restricted enzymes respectively, and linked directionally. Constructed plasmid was named pGVP, and transformated into Agrobacterium. Then, Agrobacterium-mediated leaf discs genetic transformation was carried out, and LcVP1 had been integrated into the genome of the transgenic Populus tomentosa. Carr.