Abstract:

In order to gain the RNA interference (RNAi) transformation vector of Lily, two fragment genes about 400 bp of LSV and LMoV were obtained from the total RNA of Lily leaves infected. LSV-LMoV fusion gene was constructed by recombinant PCR technique. Using Gateway cloning technology, the RNAi transformation vector pH7GWIWG2 (Ⅱ) was constructed by BP and LR recombination reactions. PCR and sequencing analysis confirmed that the pH7GWIWG2 (Ⅱ)-LSV-LMoV vector, a binary vector containing two different virus genes, was obtained successfully. Virus susceptible Lily was transformed with pH7GWIWG2 (Ⅱ)-LSV-LMoV by Agrobacterium tumefaciens-mediated transformation.