Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (4): 148-152.

Special Issue: 园艺

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ISSR Analysis of Cultivated Medicinal Species of Dendrobium Sw.

  

  • Received:2010-08-03 Revised:2010-10-12 Online:2011-02-10 Published:2011-02-10

Abstract:

To provide the reference for variety selection and cross breeding, the genetic diversity of Dendrobium was analyzed by ISSR markers. Fourteen ISSR primers were screened for the ISSR-PCR reaction, 179 polymorphic loci were detected, and the polymorphic percentage was 94.71%. Jaccard similarity index showed that the similarity index among D. candidum was 0.264-0.536, 0.351-0.561 among D. devonianum and 0.115-0.371 among different species. The result of UPGMA analysis showed it had no obvious cross breeding between intraspecies and interspecies. The ISSR results indicated the genetic diversity of Dendrobium plants from different areas was rich.