Abstract:

In previous study, the full length DNA and cDNA of the RdGASA4-like gene had been isolated from Damask rose（Rosa damascena）through homology cloning combined with rapid amplification of cDNA ends(RACE). In order to further understand the role of RdGASA4-like in the regulating network on plant development of gibberellic acid, its 685bp 5’-UTR and partial sequence upstream ATG of RdGASA4-like was obtained by Thermal Asymmetric Interlaced Polymerase Chain Reaction (TAIL-PCR). Based on sequence analysis, it was fused to a uid A gene to construct plant expression vector, named as pBI-GP685. By agrobacterium-mediated method, the explants of petiole with cotyledon of Cabbage were transformed. The transient expression study showed that the obtained promoter of RdGASA4-like was active and can drive the uid A by GUS assay analysis.