Abstract:

To identify anther abortion period and character of 373S, Electronic Microscope was employed to observe the microspore genesis difference between the male sterile line 373S and fertile line at subcellular level. The result showed that anther abortion started from the pollen mother cells stage. Cytoplasm of pollen mother cell was shrinking and no clear karyotheca was observed; At tetrad stage, a lot of small vacuolar were found in the microspore, and the subcellular structure was not clear. Endoplasmic reticulum was widely distributed in tapetum cell; At uninucleate stage. When pollen was developing, pollen wall thicken normally, but cytoplasm was obviously concentrated, endoplasmic reticulum was widely distributed in the pollen cytoplasm, but Golgi complex was rare or even disappear. Along the microspore genesis, degeneration of karyotheca was always found. No big vacuole was formed at the monokaryotic stage. In the tapetum cells, the endoplasmic reticulum was widely distributed, and Golgi complex was decreased in its number, however, the mitochondrial abnormalities were not observed. At mature stage, only hollowed pollen was left.