Abstract:

The seed of mini-papaya soaked by the different reagents (aseptic water, GA3 and 6-BA) further purified in conditional culture medium, the effect of the different reagents on germination, the length of germ and radical, the height of the seedling of the seeds were preliminary studied, and the best seed pretreatment method on the construction of the aseptic strain of the tissue culture of mini-papaya was finally screened. The result indicated that: in the condition of aseptic strain, the seeds removed from the mini-papaya which the surface of the peel uniformly wiped by 75% alcohol, rinsed by aseptic water three times was the best sterilization methods, pollution rate of the seed was 2.52%. The seeds after sterilizing soaked by the equal-volume mix-solution of GA3 1000 mg/L and 6-BA 1 mg/L for 18 h, further purified in MS culture medium, the germination, the length of germ and radical, the height of the seedling of the seeds arrived the higher value, was 68.42%, 2.25 cm, 0.8 cm, 1.52 cm respectively. The total situation of seedling growth was better, that had constructed the aseptic strain of the tissue culture of mini-papaya.