Abstract:

Effects of the stem-apex size and different combinations among 6-BA, NAA, IBA and KT on shoot tip culture in vitro were studied by using red globe grape infected with GLRaV-3 virus. GLRaV-3 was detected early in some of red globe grape of plantlets in vitro with RT-PCR technique. The results showed that the best suitable initial medium was 1/2MS+6-BA 0.5 mg/L+ KT 0.5 mg/L+NAA 0.2 mg/L. The optimum medium for shoot differentiation was B5+KT 1.0 mg/L+NAA0.2 mg/L. Plants free of GLRaV-3 were found. The size of excised shoot tip was proportional to the survival sate and inverse ratio to the virus elimination rate. The RT-PCR detection technology was used for the GLRaV-3 detection and a 300 bp specific fragment was effectively amplified. The virology detection showed that virus-free red globe grape were obtained in 28 and the average rate of virus elimination was 43%.